5. A INAH density of blood

In order to clarify the above mentioned problem, I tried a minor experimnt by setting a supersonic-wave generator in a test tube containing testing blood. The test tube was inserted in a. centrifugal separator. In this case, fortunately, I could obtain a suitable high frequency that would not cause haemolysis of the erythrocytes. It was my intention to operate the centrifugal separator, vibrate the erythrocytes, separate the minute particles adsorbed by the erythrocytes, and obtain the particles mixed with serum in the skim of the blood and observe them. The erythrocytes having heavy density may precipitate rapidly, and I expected the success of this experiment.

I obtained the serums employing above method of experiment, and other serum using the same method but not applying the supersonic--waves. I compared those two groups of serums and tried to find the difference between them.

Another method I tried was to obtain the serums of different temperatures: namely. 37°C and 0°G by using the centrifugal separator. Compared the two groups of serums and tried to find the difference between.

The above methods require comparatively large amounts of power and I called them "Large power method". I continued the experiment of the large power method from 1959 to 1965, however I could not obtain any significant result during the course of my serum chemistry experiments.

In August 1965, I prepared 10 cc of Ringer solution and mixed INAH into it so that INAH density would be exactly 9(microgram). This density is considered to be equivalent to INAH density of the blood obtained from a person who has taken 0.2 gram of INAH and passed two hours.

I prepared 1cc of erythrocytes porridge, washed it in Ringer solution for 3 times, and threw it into above mentioned 10cc mixture. Immediately the INAH density of the mixture dropped from 9r to 7r .

What is the significance of this phenomenon?

I considered it is a proof that a certain ammmt of was adsorbed by the erythrocytes.

In order to give the erythrocytes more chance of contacting, I inserted a test tube which contained the mixture into the shaking apparatus. The shaking apparatus was made to move to and fro for the distance of approximately 5 cm, several times per second so that it does not give a large amount of energy to the mixture. I called this experiment "Low power method experiment."

The result was surprising as shown on Fig 6 [not given]. I had expected some decrease of INAH density. To the contrary, it was increasing in almost straight line or directly proportional to the number of shalcing. Why? It was very interesting to me.

Here I considered that erythrocytes have special characteristics of physical bonding as well as chemical bonding similar to the phenomenon that oxygen(0₂) is being taken in by the living organisms. The small particles that were unable to be separated from the erythrocytes by the large energy method were easily separated and/or adsorbed by the application of the low power method test.

Then what happened in the test tube when it was shaken gently? Why he small energy given by the slow shaking is more effective than the vigorous vibration (Supersonic-waves) to separate small particles of DIAH from the erythrocytes? I made following assumption:

At first, oxygen was adsorbed by the erythrocytes and, in exchange, some other particles were expelled or released from the erythrocytes simultaneously.

This phenomenon is quite similar to that of oaqrgen being taken in by the living organisms. This assumption can be assured when we consider the function of the cell membrane as basis of the discussion and it will give some suggestion for the experiment of radioactivity which will be discussed later.

The erythrocytes are small blood cells that have no nucleus, and are convenient for the study of physical chendstry and adhesive and adsorptive force of small corpuscles.